Coding

Part:BBa_K4973000:Design

Designed by: Dimitrios Nikolaos Kapiris   Group: iGEM23_PatrasBio   (2023-10-06)


THI->Tachyplesin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has hemoletic action, so we added GFP protein in the composite parts, so we make it fluoscent and being able to see it with blue ligt and protect ourselves.


Source

We took the aminoacid sequence of Tachyplesin from (Panteleev PV, Ovchinnikova TV, 2017) and turmed it to nucleotide sequence with the reverse translate tool at SnapGene.

References

Panteleev PV, Ovchinnikova TV. Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity. Biotechnol Appl Biochem. 2017 Jan;64(1):35-42. doi: 10.1002/bab.1456. Epub 2015 Dec 22. PMID: 26549611.